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rhil 21 r d systems 8879 il cf  (R&D Systems)


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    Structured Review

    R&D Systems rhil 21 r d systems 8879 il cf
    Rhil 21 R D Systems 8879 Il Cf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+21+r+d+systems+8879+il+cf/Recombinant+Human+IL-21+Protein%2C+CF/us12378526-348-174-175
    Average 93 stars, based on 19 article reviews
    rhil 21 r d systems 8879 il cf - by Bioz Stars, 2026-09
    93/100 stars

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    Concentration Assay:

    Article Title: Composition for culturing natural killer cells and method for preparing natural killer cells by using same
    Article Snippet: .. TABLE 1 Basal culture medium 1 Components Manufacturer Cat. # Dose Final concentration CTS TM Immune Thermo A2596102 0.5 ml 5% Cell SR (Serum replacement) GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhiL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 2 Basal culture medium 2 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 3 Basal culture medium 3 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL X-VIVO TM 15 Lonza 04-418Q to 50 ml Medium TABLE 4 Basal culture medium 4 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% NK MACS Miltenyi Biotec 130-113-102 500 1% supplement anti-CD2/ Miltenyi Biotec 130-094-483 CD335 beads OKT3 Biolegend 317326 10 ng/mL NK MACS 130-112-968 to 50 ml medium TABLE 5 Adding material Classification Components Manufacturer Dose Final concentration Adding GI-101 GI-Innovation adding 1.6 nM/50 nM condition 1 immediately before use Adding GI-101WT GI-Cell adding 1.6 nM/50 nM condition 2 immediately before use Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 3 Fc-IL2WT immediately Fc-IL2WT (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2WT (50 nM) Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 4 Fc-IL2v2 immediately Fc-IL2v2 (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2v2 (50 nM) ..

    Magnetic Cell Separation:

    Article Title: Composition for culturing natural killer cells and method for preparing natural killer cells by using same
    Article Snippet: .. TABLE 1 Basal culture medium 1 Components Manufacturer Cat. # Dose Final concentration CTS TM Immune Thermo A2596102 0.5 ml 5% Cell SR (Serum replacement) GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhiL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 2 Basal culture medium 2 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL AIM-V medium GIBCO 0870112-DK to 50 ml TABLE 3 Basal culture medium 3 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% GLUTAMAX GIBCO A12860-01 0.5 ml 1X 100X rhIL-15 R&D systems 247-ILB/CF adding 66.7 IU immediately before use rhIL-21 R&D systems 8879-IL/CF adding 0.341 IU immediately before use anti-CD2/ Miltenyi Biotec 130-094-483 CD335 Beads OKT3 Biolegend 317326 10 ng/mL X-VIVO TM 15 Lonza 04-418Q to 50 ml Medium TABLE 4 Basal culture medium 4 Components Manufacturer Cat. # Dose Final concentration Human AB serum Sigma H4522 2.5 ml 5% NK MACS Miltenyi Biotec 130-113-102 500 1% supplement anti-CD2/ Miltenyi Biotec 130-094-483 CD335 beads OKT3 Biolegend 317326 10 ng/mL NK MACS 130-112-968 to 50 ml medium TABLE 5 Adding material Classification Components Manufacturer Dose Final concentration Adding GI-101 GI-Innovation adding 1.6 nM/50 nM condition 1 immediately before use Adding GI-101WT GI-Cell adding 1.6 nM/50 nM condition 2 immediately before use Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 3 Fc-IL2WT immediately Fc-IL2WT (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2WT (50 nM) Adding CD80-Fc + GI-Cell adding CD80-Fc (1.6 nM) + condition 4 Fc-IL2v2 immediately Fc-IL2v2 (1.6 nM)/ before use CD80-Fc (50 nM) + Fc-IL2v2 (50 nM) ..



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    93
    R&D Systems rhil 21 r d systems 8879 il cf
    Rhil 21 R D Systems 8879 Il Cf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhil+21+r+d+systems+8879+il+cf/Recombinant+Human+IL-21+Protein%2C+CF/us12378526-348-174-175
    Average 93 stars, based on 1 article reviews
    rhil 21 r d systems 8879 il cf - by Bioz Stars, 2026-09
    93/100 stars
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